Real Time PCR Assays and Arrays
Biotium CD71 / Transferrin Receptor (TFRC)(TFRC/1396), CF594 conjugate, 0.1mg/mL
Recognizes a ~90-95 kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190 kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescenc
AURORA BIOLABS LLC Kras G12V Binding Assay, 384 rxn
The Aurora Biolabs KRAS G12V c-RAF Binding Assay Kit is a TR-FRET based assay used to quantify binding between active KRAS G12V and the Ras Binding Domain (RBD) of c-RAF. The KRAS protein is preloaded with GTP and recognized by a terbium-labeled anti-Tag2 antibody (HTRF donor), while the c-RAF-RBD is labeled with a fluorescent acceptor. Upon binding, FRET occurs and emission at 665nm is measured. This assay provides a robust method for screening inhibitors that block KRAS effector interactions. Ideal for oncology research and drug discovery, each kit contains reagents for 384 reactions.
AURORA BIOLABS LLC Kras Q61R Nucleotide Exchange Assay Kit,384 rxn
KRAS is a member of the RAS protein family, a group of small GTPases involved in cell signaling pathways that regulate cell growth, proliferation, differentiation, and survival. KRAS functions as a molecular switch by cycling between an inactive GDP-bound state and an active GTP-bound state. Activation of KRAS triggers downstream signaling pathways that promote normal cellular functions. Mutations in KRAS, such as the Q61R mutation, impair its GTPase activity and lock the protein in the active GTP-bound form, resulting in continuous signaling that drives uncontrolled cell proliferation and cancer development. KRAS mutations are frequently found in pancreatic, colorectal, and lung cancers. The KRAS Q61R nucleotide exchange assay is a TR-FRET–based assay designed to measure GTP binding activity and evaluate inhibitors targeting KRAS Q61R-mediated signaling pathways.
AURORA BIOLABS LLC Kras G12A, GST-tag,100
Kras is a member of the RAS protein family, which are a class of small GTPases involved in cell signaling. Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner. The protein also contains mutation of interested G12A.
STEMCELL Technologies Human Pluripotent Stem Cell Trilineage Differentiation qPCR Array, Size: 1 Unit
For characterization of hPSC trilineage differentiation to endoderm mesoderm and ectoderm germ layers
Biotium CD71 / Transferrin Receptor (TFRC) (Extracellular Domain) (TFRC/1817), CF405S conjugate, 0.1mg/mL
This antibody recognizes a ~90-95 kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190 kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lowe
Genscript Corporation KRAS His Human G12D
The KRAS gene provides instructions for making a protein called K-Ras part of the RAS/MAPK pathway The protein relays signals from outside the cell to the cell s nucleus These signals instruct the cell to grow and divide (proliferate) or to mature and take on specialized functions (differentiate) The K-Ras protein is a GTPase which means it converts a molecule called GTP into another molecule called GDP In this way the K-Ras protein acts like a switch that is turned on and off by the GTP and GDP molecules KRAS is usually tethered to cell membranes because of the presence of an isoprene group on its C-terminus There are two protein products of the KRAS gene in mammalian cells that result from the use of alternative exon 4 (exon 4A and 4B respectively) K-Ras4A and K-Ras4B these proteins have different structure in their C-terminal region and use different mechanisms to localize to cellular membranes including the plasma membrane
AURORA BIOLABS LLC Kras Q61R–cRAF Binding Assay Kit,100 rxn
KRAS is a small GTPase in the RAS protein family that regulates key cellular processes such as proliferation, differentiation, and survival through the RAS-RAF signaling pathway. Activation of KRAS occurs when GDP is exchanged for GTP, enabling interaction with downstream effectors including cRAF. Mutations in KRAS are frequently associated with human cancers, making the KRAS-cRAF interaction an important target for therapeutic development. The KRAS (Q61R)-cRAF Binding Assay Kit is a TR-FRET–based assay designed to measure the interaction between activated KRAS and cRAF. In this assay, Tag2-KRAS (Q61R) is loaded with GppNHp to mimic the active GTP-bound state, while Tag1-cRAF RBD binds activated KRAS. Binding generates a FRET signal detected by fluorescence emission at 665 nm and 620 nm. Inhibitors that disrupt KRAS-cRAF binding reduce the HTRF signal intensity.
AURORA BIOLABS LLC Kras G13D, GST-tag,100
Kras is a member of the RAS protein family, which are a class of small GTPases involved in cell signaling. Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner. The protein also contains mutation of interested G13D.
American Research Products Inc PrEST Antigen ABR
PrEST Antigen ABR antigen APrEST85626 for active BCR-related Recombinant protein fragment of Human ABR